Observations on an Isolated, Innervated Preparation of Rat Urinary Bladder

Preparations of isolated tissues together with their extrinsic nerves are useful for obtaining information about the autonomic innervation and the mode of action of various drugs. Ursillo & Clark (1956) described a preparation of rabbit bladder consisting of a strip of the detrussor muscle together with its periarterial nerves, and Burnstock & Campbell (1963) studied the whole isolated bladder of the marsupial possum together with the periarterial nerves accompanying the blood vessels to the urethra and to the fundus. The isolated innervated preparation of the rat urinary bladder has been used previously for the study of drug action (Bobarevic, Hukovic & Dezeli6, 1962; Hukovic & Bubic, 1963) and it was demonstrated to the British Pharmacological Society by Hukovic, Rand & Vanov in 1964. This paper gives a further description of the preparation and its responses to drugs with reference to the autonomic innervation of the bladder.