May 1, 1973European Journal of Biochemistry

Purification of a Messenger‐Specific Initiation Factor from Ascites‐Cell Supernatant

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Population

Krebs II ascites cells

Design

Preclinical

Authors

DWDonald T. WigleHealth Canada

Discussion

Member takes

Implication

Hypothesis-generating in animal models; leaves open any cardiovascular relevance.

Structured PICO

Population

Krebs II ascites cells

Intervention

Purification process involving (NH4)2SO4 fractionation followed by chromatography on hydroxyapatite, DEAE-Sephadex A-50, Sephadex G-100 superfine and phosphocellulose

Outcome

Purification to homogeneity of a supernatant initiation factor required for the initiation of translation of encephalomyocarditis virus RNAsurrogate

The study successfully purified a messenger-specific initiation factor from ascites cells, determining its molecular weight to be approximately 53,000.

Cite This Study

Donald T. Wigle (1973) studied this question.

synapsesocial.com/papers/6a703a2be36a167817e1fb84 https://doi.org/10.1111/j.1432-1033.1973.tb02803.x

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Purification of a Messenger‐Specific Initiation Factor from Ascites‐Cell Supernatant

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Purification of a Messenger‐Specific Initiation Factor from Ascites‐Cell Supernatant

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider