What question did this study set out to answer?

The research aims to enhance the purification and concentration of encephalomyocarditis virus using various techniques.

September 1, 1969Journal of General Virology

Purification of Encephalomyocarditis Virus

Q: What does this research mean for the field?

Encephalomyocarditis virus can be concentrated over 1000-fold and highly purified using a combination of acid precipitation, organic solvent extraction, enzyme treatment, and differential centrifugation, yielding homogeneous polyhedral particles. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

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Population

Encephalomyocarditis virus (crude virus preparation)

Design

Preclinical

Authors

ABA. T. H. BurnessMemorial University of Newfoundland

Discussion

Member takes

Implication

May aid EMCV research models; leaves open translation to human cardiovascular applications.

Key Points

The research aims to enhance the purification and concentration of encephalomyocarditis virus using various techniques.
Combined acid precipitation, organic solvent extraction, enzyme treatment, and differential centrifugation.
Estimated yield using 32P-labeled virus and plaque forming units.
Determined purification degree by assessing contamination with labeled host-cell material.
Achieved a concentration more than 1000-fold with a yield of 30% to 50%.
The degree of purification exceeded 1 × 10^4.
Final product exhibited a homogeneous preparation with a particle to plaque forming units ratio of 250 ± 40.

Structured PICO

Population

Encephalomyocarditis virus (crude virus preparation)

Intervention

Purification techniques including acid precipitation, organic solvent extraction, enzyme treatment, and differential centrifugation

Outcome

Concentration, yield, and degree of purification of the virussurrogate

A combination of techniques achieved a >1000-fold concentration and >10^4 degree of purification of encephalomyocarditis virus with 30-50% yield.

Cite This Study

A. T. H. Burness (1969) studied this question.

synapsesocial.com/papers/6a1c0d791567d2fc4d5f82ac https://doi.org/10.1099/0022-1317-5-2-291

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September 1, 1969Journal of General Virology

Purification of Encephalomyocarditis Virus

View Full Paper

Ask AI

Bookmark

Population

Encephalomyocarditis virus (crude virus preparation)

Design

Preclinical

Authors

ABA. T. H. BurnessMemorial University of Newfoundland

Discussion

Member takes

Implication

May aid EMCV research models; leaves open translation to human cardiovascular applications.

Key Points

The research aims to enhance the purification and concentration of encephalomyocarditis virus using various techniques.
Combined acid precipitation, organic solvent extraction, enzyme treatment, and differential centrifugation.
Estimated yield using 32P-labeled virus and plaque forming units.
Determined purification degree by assessing contamination with labeled host-cell material.
Achieved a concentration more than 1000-fold with a yield of 30% to 50%.
The degree of purification exceeded 1 × 10^4.
Final product exhibited a homogeneous preparation with a particle to plaque forming units ratio of 250 ± 40.

Structured PICO

Population

Encephalomyocarditis virus (crude virus preparation)

Intervention

Purification techniques including acid precipitation, organic solvent extraction, enzyme treatment, and differential centrifugation

Outcome

Concentration, yield, and degree of purification of the virussurrogate

A combination of techniques achieved a >1000-fold concentration and >10^4 degree of purification of encephalomyocarditis virus with 30-50% yield.

Cite This Study

A. T. H. Burness (1969) studied this question.

synapsesocial.com/papers/6a1c0d791567d2fc4d5f82ac https://doi.org/10.1099/0022-1317-5-2-291

Also Consider

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