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April 1, 1985Avian Diseases

A Rapid Procedure for the Purification of Avian Encephalomyelitis Viruses

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Key result

A rapid purification procedure using trichlorotrifluorethane, polyethylene glycol, and 125iodine labeling successfully yielded avian encephalomyelitis virus free of tissue debris.

Population

Egg-grown or field preparations of avian encephalomyelitis virus (AEV) of neural origin

Design

Preclinical

Authors

GTG. A. TannockThe Royal Melbourne HospitalDSDarren R. ShafrenCalvary Mater Newcastle Hospital

Discussion

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Overview

May expedite AEV lab protocols; leaves open translation to clinical or cardiovascular research.

Structured PICO

P
Population
Egg-grown or field preparations of avian encephalomyelitis virus (AEV) of neural origin
I
Intervention
Rapid purification procedure involving clarification, partial purification with trichlorotrifluorethane (Freon TF), concentration with polyethylene glycol, re-extraction with Freon, 125iodine labeling, and final purification by cesium-chloride equilibrium or sucrose-velocity-gradient centrifugation
O
Outcome
Purity and characteristics of the isolated virus

The described rapid purification procedure effectively isolates avian encephalomyelitis virus free of tissue debris for further virological study.

Cite This Study

Tannock et al. (1985) studied Avian encephalomyelitis virus (AEV). Rapid purification procedure was evaluated on Virus purification and detection. A rapid purification procedure using trichlorotrifluorethane, polyethylene glycol, and 125iodine labeling successfully yielded avian encephalomyelitis virus free of tissue debris.

synapsesocial.com/papers/6ab475c765c63ad2bccc976bhttps://doi.org/10.2307/1590491
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus2005 · 29 citations
  2. 2Purification of Encephalomyocarditis Virus1969 · 25 citations
  3. 3PURIFICATION OF JAPANESE ENCEPHALITIS VIRUS AND ITS ANTIGENIC PARTICLES FROM INFECTED SUCKLING MOUSE BRAINS1964 · 6 citations
  4. 4Measurement of antibodies to infectious avian encephalomyelitis virus by elisa1985 · 10 citations
  5. 5Structural Studies on Avian Myeloblastosis Virus: Rapid Purification and Quantitation1972 · 5 citations