Key result
RT-PCR specifically detected avian encephalomyelitis virus from chicken embryo brain at 3 days postinoculation, compared with up to 11 days required for the agar gel precipitation test.
Population
Avian encephalomyelitis virus (5 reference/field strains) and chicken embryo brain models
Comparison
Reverse transcriptase-polymerase chain reaction… vs Avian encephalomyelitis agar gel precipitation…
Design
Preclinical
Authors
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RT-PCR enables earlier AE virus detection in embryos; leaves open its diagnostic role pending field validation.
A newly developed RT-PCR assay for avian encephalomyelitis virus is highly sensitive and specific, allowing for significantly faster detection than the standard agar gel precipitation test.
Xie et al. (2005) studied Avian encephalomyelitis virus (AEV). Reverse transcriptase-polymerase chain reaction (RT-PCR) vs. Agar gel precipitation test (AGP) was evaluated on Detection of avian encephalomyelitis virus. RT-PCR specifically detected avian encephalomyelitis virus from chicken embryo brain at 3 days postinoculation, compared with up to 11 days required for the agar gel precipitation test.
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