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January 1, 2016Veterinary WorldOpen Access

Analysis of viral protein-2 encoding gene of avian encephalomyelitis virus from field specimens in Central Java region, Indonesia

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Population

13 virus samples isolated from various species of poultry serologically diagnosed as infected by Avian…

Design

Preclinical

Authors

AHAris HaryantoUniversitas Gadjah MadaRERatna ErmawatiLampung UniversityVWVera WatiInstitute for Women Human Rights

Discussion

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Implication

VP-2 gene sequencing from field specimens supports expanded AEV surveillance in poultry; leaves open strain diversity and vaccine implications.

Structured PICO

P
Population
13 virus samples isolated from various species of poultry (layer, duck, broiler) serologically diagnosed as infected by Avian encephalomyelitis virus (AEV) from Central Java, Indonesia.
I
Intervention
Reverse transcription polymerase chain reaction (RT-PCR) amplification of the viral protein 2 (VP-2) encoding gene.
O
Outcome
Detection of the VP-2 encoding gene of AEV (DNA fragment size 619 bp) and sensitivity of the RT-PCR method.surrogate

RT-PCR targeting the VP-2 gene is a sensitive and specific method for the rapid detection of avian encephalomyelitis virus in field specimens.

Limitations

  • Did not have field samples from young birds of less than 4 weeks

Cite This Study

Haryanto et al. (2016) studied this question.

synapsesocial.com/papers/6a71ffbeb5c1fe5ca9dfee02https://doi.org/10.14202/vetworld.2016.25-31
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Avian encephalomyelitis: A review1994 · 46 citations
  2. 2Avian encephalomyelitis virus in reared pheasants: a case study2009 · 32 citations
  3. 3Avian encephalomyelitis virus is a picornavirus and is most closely related to hepatitis A virus.1999 · 101 citations
  4. 4The Picornavirus Avian Encephalomyelitis Virus Possesses a Hepatitis C Virus-Like Internal Ribosome Entry Site Element2007 · 52 citations
  5. 5Chickens host diverse picornaviruses originated from potential interspecies transmission with recombination2014 · 37 citations