Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
April 1, 1999Avian Diseases

Characterization of the Genome of Avian Encephalomyelitis Virus with Cloned Cdna Fragments

View Full Paper
Ask AI
Bookmark
Share

Key result

Avian encephalomyelitis virus genome spans ~7.5 kb with close homology to hepatitis A virus.

Why the study?

The genome of avian encephalomyelitis virus (AEV) was not fully characterized, limiting diagnostic method development.

Population

RNA extracted from avian encephalomyelitis virus preparations

Comparison

RT-PCR and RACE amplification vs viral RNA

Design

Molecular cloning and sequence analysis study

Authors

DTD. ToddUniversity of North Carolina at GreensboroJWJ H WestonKMK. A. MawhinneyUniversity of Ulster

Discussion

Loading...

Member takes

Overview

AEV cDNA cloning via RT-PCR supports picornavirus classification; leaves open full genome and pathogenesis studies.

Structured PICO

P
Population
Avian encephalomyelitis virus (AEV) RNA preparations
I
Intervention
Reverse transcription-polymerase chain reaction (RT-PCR), rapid amplification of cDNA ends (RACE), cloning, and sequence analysis
O
Outcome
Characterization of the AEV genome (size, polyadenylation status, and sequence homology)

The characterization of the AEV genome reveals close homology to hepatitis A virus, suggesting it should be reassigned to a different genus within the Picornaviridae family and providing targets for diagnostic development.

Cite This Study

Todd et al. (1999) studied Avian encephalomyelitis virus (AEV). cDNA cloning and sequence analysis was evaluated on Characterization of the AEV genome. Sequence analysis of the avian encephalomyelitis virus genome revealed a polyadenylated, single-stranded RNA of ~7.5 kb with close homology to hepatitis A virus.

synapsesocial.com/papers/6ab475c765c63ad2bccc976ahttps://doi.org/10.2307/1592611
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Avian encephalomyelitis virus is a picornavirus and is most closely related to hepatitis A virus.1999 · 101 citations
  2. 2Reverse Transcriptase-Polymerase Chain Reaction to Detect Avian Encephalomyelitis Virus2005 · 29 citations
  3. 3Further Evidence That the Nucleic Acid of Avian Encephalomyelitis Virus Consists of RNA1992 · 9 citations
  4. 4Analysis of viral protein-2 encoding gene of avian encephalomyelitis virus from field specimens in Central Java region, Indonesia2016 · 11 citations
  5. 5Characterization of Avian Encephalomyelitis Virus (An Avian Enterovirus)1969 · 36 citations