A Rapid Method for the Purification of the MEFj Strain of Poliomyelitis Virus

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Population

Infected brain suspensions containing the MEFj strain of poliomyelitis virus

Design

Preclinical

Authors

Discussion

May enable efficient preclinical virus purification; leaves open translation to other models or clinical use.

Population

Infected brain suspensions containing the MEFj strain of poliomyelitis virus

Intervention

Purification method involving dialysis at pH 4.3, redispersion at pH 8.2, chloroform shaking, and ultracentrifugation

Outcome

Recovery of the virus in a relatively pure state quantitativelysurrogate

Describes a rapid method for purifying the MEFj strain of poliomyelitis virus from infected brain suspensions.

A. Poison (1993) studied this question.

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