Key result
Centrifugation for four hours at 18,000 r.p.m. allowed quantitative recovery of poliomyelitis virus from a 20% saline suspension, or a 1% suspension with 10% normal serum added.
Population
Poliomyelitis virus in saline suspensions (20% and 1%) and human stools (n=4)
Design
Preclinical
Authors
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May aid poliomyelitis virus concentration from dilute samples; leaves open validation for clinical stool diagnostics.
A centrifugation technique at 18,000 r.p.m. for four hours successfully sediments poliomyelitis virus for quantitative recovery.
Rosalie J. Silverberg (1945) studied Poliomyelitis virus. Centrifugation was evaluated on Quantitative recovery of virus in the sediment. Centrifugation for four hours at 18,000 r.p.m. allowed quantitative recovery of poliomyelitis virus from a 20% saline suspension, or a 1% suspension with 10% normal serum added.
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