Key result
Pervaporation of large quantities of tissue culture fluid containing approximately 10^6.5 T.C.I.D.50 per ml yielded concentrated virus material with titres of the order of 10^10 T.C.I.D.50 per ml.
Pervaporation and dialysis can be used to concentrate polio virus from tissue culture fluids to high titres for immunogenic potency studies.
May enable high-titre polio virus concentrates for research; leaves open applicability to contemporary immunogenic studies.
For the purpose of determining the immunogenic potency of polio virus, relatively large amounts of concentrated virus material were prepared which had titres of the order of 1010 T.C.I.D.50 per ml. These were obtained by pervaporating large quantities of tissue culture fluid containing approximately 106.5 T.C.I.D.50 per ml. We wish to acknowledge the valuable assistance received from Dr P. D. Winter and his staff, of the Poliomyelitis Research Foundation, in carrying out the tissue culture titrations of these materials.
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Polson et al. (1957) studied Poliomyelitis virus. Pervaporation and dialysis was evaluated on Virus titre. Pervaporation of large quantities of tissue culture fluid containing approximately 10^6.5 T.C.I.D.50 per ml yielded concentrated virus material with titres of the order of 10^10 T.C.I.D.50 per ml.
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