What question did this study set out to answer?

To accurately quantify the ribonucleic acid (RNA) content and determine specific extinction coefficients of encephalomyocarditis virus.

March 1, 1970Journal of General Virology

Ribonucleic Acid Content of Encephalomyocarditis Virus

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ABA. T. H. BurnessMemorial University of Newfoundland

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Implication

Biochemical analysis demonstrates an RNA content of 31.7% in encephalomyocarditis virus, highlighting precise composition values for viral characterization.

Key Points

To accurately quantify the ribonucleic acid (RNA) content and determine specific extinction coefficients of encephalomyocarditis virus.
Quantified RNA content using nitrogen and phosphorus determinations, orcinol colorimetric assays, and ultraviolet extinction measurements at 260 nm on perchloric acid hydrolysates.
Measured specific extinction coefficients (E 1% 1 cm) at 260 nm for intact virus particles in KCl-phosphate buffer (pH 8.0) and for isolated viral RNA in Tris-HCl buffer (pH 7.2).
The overall RNA content of encephalomyocarditis virus was established at 31.7%, derived from nitrogen and phosphorus analyses (32.0%), perchloric acid hydrolysate extinction (31.3%), and orcinol determinations (31.8%).
Specific extinction coefficients at 260 nm were determined to be 77.4 for intact virus in 0.1 M KCl with 0.02 M phosphate buffer (pH 8.0) and 221 for isolated viral RNA in Tris-HCl (pH 7.2).

Cite This Study

A. T. H. Burness (1970) studied this question.

synapsesocial.com/papers/6a8918693c66b321f60c430c https://doi.org/10.1099/0022-1317-6-3-373

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Implication

Biochemical analysis demonstrates an RNA content of 31.7% in encephalomyocarditis virus, highlighting precise composition values for viral characterization.

Ribonucleic Acid Content of Encephalomyocarditis Virus

Discussion

Member takes

Implication

Key Points

Cite This Study

Ribonucleic Acid Content of Encephalomyocarditis Virus

Discussion

Member takes

Implication

Key Points

Cite This Study

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