What question did this study set out to answer?

To characterize the molecular and functional remodeling of voltage-gated sodium channels in mouse cardiomyocytes across distinct embryonic developmental stages.

January 1, 2011Circulation JournalOpen Access

Molecular and Functional Changes in Voltage-Gated Na+ Channels in Cardiomyocytes During Mouse Embryogenesis

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Authors

LYLiangzhu YuHubei University of Science and Technology SGShijun GaoJohns Hopkins University LNLi NieThird Affiliated Hospital of Southern Medical University

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Implication

Experimental study reveals distinct developmental shifts in sodium channel kinetics and subunit expression in embryonic mouse cardiomyocytes, highlighting their essential role in heart maturation.

Key Points

To characterize the molecular and functional remodeling of voltage-gated sodium channels in mouse cardiomyocytes across distinct embryonic developmental stages.
Recorded whole-cell sodium currents in early developmental stage (EDS) and late developmental stage (LDS) cardiomyocytes isolated from mouse embryos.
Profiled the gene expression of sodium channel alpha-subunits (Nav 1.1–1.6) and beta-subunits (Nav beta1–beta3) and determined pharmacological sensitivity using tetrodotoxin (TTX).
Compared to EDS myocytes, LDS myocytes displayed larger peak current density, a hyperpolarizing shift in half-inactivation voltage, and significantly faster recovery kinetics from inactivation.
Expression levels of Nav 1.4–1.6 and Nav beta1–beta3 transcripts increased during embryogenesis, whereas Nav 1.1–1.3 transcripts remained absent or minimal.
Tetrodotoxin sensitivity assays identified the TTX-resistant Nav 1.5 subunit as the predominant functional sodium channel isoform in the developing embryonic heart.

Cite This Study

Yu et al. (2011) studied this question.

synapsesocial.com/papers/6a7040a926a7f98052dcc072 https://doi.org/10.1253/circj.cj-10-1212

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Implication

Experimental study reveals distinct developmental shifts in sodium channel kinetics and subunit expression in embryonic mouse cardiomyocytes, highlighting their essential role in heart maturation.

Molecular and Functional Changes in Voltage-Gated Na+ Channels in Cardiomyocytes During Mouse Embryogenesis

Discussion

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Implication

Key Points

Cite This Study

Molecular and Functional Changes in Voltage-Gated Na+ Channels in Cardiomyocytes During Mouse Embryogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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