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August 1, 1992Journal of Clinical InvestigationOpen Access

Polymerase chain reaction localization of constitutive nitric oxide synthase and soluble guanylate cyclase messenger RNAs in microdissected rat nephron segments.

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Population

Rat kidney

Design

Preclinical

Authors

YTYoshio TeradaKochi Medical School HospitalKTKimio TomitaTsurugi HospitalHNHiroshi NonoguchiNational Sagamihara Hospital

Discussion

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Implication

Maps intrarenal NO sites in rodents; leaves open translation to human renal physiology and disease.

Structured PICO

P
Population
Rat kidney (microdissected renal tubule segments along the nephron, glomeruli, vasa recta bundle, and arcuate arteries)
I
Intervention
Reverse transcription and polymerase chain reaction (RT-PCR) assay
O
Outcome
Microlocalization of mRNAs coding for constitutive NO synthase and soluble guanylate cyclasesurrogate

Demonstrates that NO can be produced locally in specific segments of the rat kidney and that its target, soluble guanylate cyclase, is widely distributed in renal structures and the vascular system.

Cite This Study

Terada et al. (1992) studied this question.

synapsesocial.com/papers/6a7070f85671bbf00bc2b3bfhttps://doi.org/10.1172/jci115908
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1RT-PCR microlocalization of mRNA for guanylyl cyclase-coupled ANF receptor in rat kidney1991 · 62 citations
  2. 2Role of endothelium-derived relaxing factor in regulation of renal hemodynamic responses1990 · 248 citations
  3. 3Atrial natriuretic factor inhibits vasopressin-stimulated osmotic water permeability in rat inner medullary collecting duct.1988 · 119 citations
  4. 4The involvement of endothelium‐derived relaxing factor in the regulation of renal cortical blood flow in the rat1991 · 98 citations
  5. 5Nitric oxide activates guanylate cyclase and increases guanosine 3′:5′-cyclic monophosphate levels in various tissue preparations1977 · 1,464 citations