What question did this study set out to answer?

To determine the relationship between the Rnr regulatory locus on Chromosome 1 and the structural gene encoding mouse submaxillary gland renin using enzyme thermostability.

January 1, 1982Journal of Biological ChemistryOpen Access

Genetic regulation of thermostability of mouse submaxillary gland renin.

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Authors

CWCarol M. WilsonBelfast Health and Social Care Trust BTBenjamin A. TaylorDalhousie University

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Implication

Clarifies Rnr-renin structural gene link in mice; leaves open translation to human hypertension genetics.

Key Points

To determine the relationship between the Rnr regulatory locus on Chromosome 1 and the structural gene encoding mouse submaxillary gland renin using enzyme thermostability.
Measured the inactivation half-life (t1/2) of submaxillary gland renin at 60 °C across 13 Rnrb strains, 24 Rnrs strains, 55 recombinant inbred mouse strains, and an Rnrs congenic strain.
Assessed renin thermostability following enzyme purification, dilution, and mixing of labile and stable forms, and tested reactivity against strain-specific alloantibodies.
Renin from 13 strains carrying the Rnrb allele showed significantly higher thermostability at 60 °C (mean t1/2 = 13.6 ± 0.9 min) than renin from 24 strains carrying the Rnrs allele (mean t1/2 = 2.1 ± 0.04 min).
All 55 recombinant inbred strains and the Rnrs congenic strain demonstrated complete concordance between the Rnrs allele and the thermolabile phenotype.
Alloantibodies elicited by Rnrs/Rnrs submaxillary gland renin selectively inhibited Rnrs renin activity but did not block Rnrb renin.

Cite This Study

Wilson et al. (1982) studied this question.

synapsesocial.com/papers/6a7080ef26770c2b8de092be https://doi.org/10.1016/s0021-9258(19)68348-1

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Genetic regulation of thermostability of mouse submaxillary gland renin.

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Key Points

Cite This Study

Genetic regulation of thermostability of mouse submaxillary gland renin.

Discussion

Member takes

Implication

Key Points

Cite This Study

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