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April 1, 1975Journal of Clinical InvestigationOpen Access

Association of tissue factor activity with the surface of cultured cells.

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Population

Cultured normal human fibroblasts and WISH 1 amnion cells

Comparison

Prolonged digestion with dilute trypsin vs Undisturbed monolayers or cells lifted with…

Design

Preclinical

Authors

JMJR MaynardCHCarol A. HeckmanFPF A Pitlick

Discussion

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Overview

Latent tissue factor in intact cells may initiate coagulation; hypothesis-generating for in vivo thrombosis mechanisms.

Structured PICO

P
Population
Cultured normal human fibroblasts and WISH 1 amnion cells
I
Intervention
Prolonged digestion with dilute trypsin (0.0025 per cent trypsin for 30 min)
C
Comparator
Undisturbed monolayers or cells lifted with brief trypsin treatment (0.125 per cent trypsin for 1 min)
O
Outcome
Tissue factor activitysurrogate

Cultured cells possess latent tissue factor activity that can be activated and released without total cell disruption, potentially initiating blood coagulation.

Cite This Study

Maynard et al. (1975) studied this question.

synapsesocial.com/papers/6a70a08d2fdcb5703edb6354https://doi.org/10.1172/jci107992
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Tissue Factor Activity in Lymphocyte Cultures from Normal Individuals and Patients with Hemophilia A1973 · 112 citations
  2. 2Binding of the protein component of tissue factor to phospholipids1970 · 49 citations
  3. 3Ecto-Enzyme of Granulocytes: 5′-Nucleotidase1974 · 107 citations
  4. 4The release of radioactive nucleic acids and mucoproteins by trypsin and ethylenediaminetetra-acetate treatment of baby-hamster cells in tissue culture1970 · 54 citations