January 1, 2000HypertensionOpen Access

Cytochrome P450 and Cyclooxygenase Metabolites Contribute to the Endothelin-1 Afferent Arteriolar Vasoconstrictor and Calcium Responses

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Why the study?

Does inhibition of CYP450 or COX attenuate endothelin-1-induced vasoconstriction and calcium responses in rat renal microvessels?

Population

Rat juxtamedullary nephron preparation and freshly isolated smooth muscle cells from preglomerular…

Comparison

Cytochrome P450 hydroxylase inhibitor… vs Endothelin-1 alone

Design

Preclinical

Authors

JIJohn D. ImigVascular Medicine BPBao Thang PhamLouisiana State University ELElizabeth A. LeBlancLouisiana State University

Discussion

Member takes

Implication

Hypothesis-generating in rat models; leaves open whether CYP450/COX inhibition offers therapeutic targets in human hypertension.

Structured PICO

Does inhibition of CYP450 or COX attenuate endothelin-1-induced vasoconstriction and calcium responses in rat renal microvessels?

Population

Rat juxtamedullary nephron preparation (n=17 afferent arterioles) and freshly isolated smooth muscle cells from preglomerular microvessels (n=42 cells)

Intervention

Cytochrome P450 (CYP450) hydroxylase inhibitor N-methylsulfonyl-12,12-dibromododec-11-enamide (DDMS), cyclooxygenase (COX) inhibitor indomethacin, or CYP450 epoxygenase inhibitor N-methylsulfonyl-6-(2-proparglyoxyphenyl) hexanamide, administered prior to endothelin-1 (ET-1)

Comparator

Endothelin-1 (ET-1) alone (baseline response before inhibition)

Outcome

Afferent arteriolar diameter and microvascular smooth muscle cell intracellular calcium concentration ([Ca(2+)](i))surrogate

CYP450 hydroxylase and COX arachidonic acid metabolites are important contributors to ET-1-induced afferent arteriolar vasoconstriction and calcium signaling.

Cite This Study

Imig et al. (2000) studied this question.

synapsesocial.com/papers/6a70a0cafe4101aa97e03ff0 https://doi.org/10.1161/01.hyp.35.1.307

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Cytochrome P450 and Cyclooxygenase Metabolites Contribute to the Endothelin-1 Afferent Arteriolar Vasoconstrictor and Calcium Responses

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Cytochrome P450 and Cyclooxygenase Metabolites Contribute to the Endothelin-1 Afferent Arteriolar Vasoconstrictor and Calcium Responses

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider