December 1, 1973Transplantation
Homing Properties of Thymus-Independent Follicular Lymphocytes
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Discussion
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Implication
Experimental animal study demonstrates selective homing of B lymphocytes to lymphoid follicles in normal recipients, indicating primary follicles serve as in vivo localization sites.
Key Points
- To investigate the tissue entry sites and in vivo homing preferences of thymus-independent follicular small lymphocytes.
- Extracted primary follicle small lymphocytes enriched to 87–94% immunoglobulin positivity from lymph nodes of B mice.
- Labeled isolated cells with 3H-uridine or chromium radioisotopes and infused them into normal recipient mice.
- Assessed in vivo cellular distribution and localization using autoradiography and total organ radioactivity measurements.
- Transfused follicular lymphocytes entered recipient lymphoid tissues through entry sites identical to those utilized by T cells.
- Follicular lymphocytes migrated selectively to primary follicles within recipient lymph nodes and spleen, preferentially avoiding thymus-dependent regions.
Cite This Study
Gutman et al. (1973) studied this question.