What question did this study set out to answer?

To evaluate the effects of acute myocardial ischemia and subsequent reperfusion on sarcoplasmic reticulum calcium-ATPase activity and contraction band formation.

January 1, 1992Japanese Circulation Journal-english EditionOpen Access

Study on reperfusion injury on sarcoplasmic reticulum in acute myocardial ischemia.

View Full Paper

Ask AI

Bookmark

Why the study?

Does reperfusion after early myocardial ischemia induce sarcoplasmic reticulum injury in a dog model?

Population

Dog model of acute myocardial ischemia

Comparison

Reperfusion for 15 minutes following 10, 20, or… vs Ischemia without reperfusion and control

Design

Preclinical

Follow-up

15 minutes

Authors

SISeiji ItohGuilford Press (United States)TYToshikuni YanagishitaShowa University SMSyuji MukaeShowa University

Discussion

Member takes

Implication

Reperfusion after brief ischemia may impair SR Ca-ATPase in dogs; leaves open translation to human myocardial protection.

Key Points

To evaluate the effects of acute myocardial ischemia and subsequent reperfusion on sarcoplasmic reticulum calcium-ATPase activity and contraction band formation.
Induced acute myocardial ischemia in canine models via left anterior descending coronary artery occlusion for 10, 20, or 30 minutes.
Administered 15 minutes of reperfusion following ischemic periods to assess subendomyocardial alterations.
Quantified sarcoplasmic reticulum Ca2+-ATPase activity, major ATPase protein composition, and morphological contraction band appearance.
Subendomyocardial Ca2+-ATPase activity decreased significantly to 60% of control after 10 minutes of ischemia followed by 15 minutes of reperfusion, whereas 10 minutes of ischemia alone maintained activity near control levels.
Ischemia for 30 minutes reduced Ca2+-ATPase activity to 60% of control, while major ATPase protein loss occurred at 30 minutes of ischemia and after only 20 minutes of reperfusion.
Contraction band appearance in the subendomyocardium increased significantly after 20 minutes or more of reperfusion.

Structured PICO

Does reperfusion after early myocardial ischemia induce sarcoplasmic reticulum injury in a dog model?

Population

Dog model of acute myocardial ischemia

Intervention

Reperfusion for 15 minutes following 10, 20, or 30 minutes of left anterior descending coronary artery (LAD) occlusion

Comparator

Ischemia without reperfusion (10, 20, and 30 minutes) and control

Outcome

Ca(++)-ATPase activity of sarcoplasmic reticulum and proportion of contraction bands in subendomyocardiumsurrogate

Reperfusion injury, characterized by reduced sarcoplasmic reticulum Ca(++)-ATPase activity and increased contraction bands, can occur after as little as 10 minutes of ischemia in a dog model.

Cite This Study

Itoh et al. (1992) studied this question.

synapsesocial.com/papers/6a70a958660549caf2c548ba https://doi.org/10.1253/jcj.56.384

View Full Paper

Ask AI

Bookmark

Study on reperfusion injury on sarcoplasmic reticulum in acute myocardial ischemia.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Study on reperfusion injury on sarcoplasmic reticulum in acute myocardial ischemia.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider