What question did this study set out to answer?

To evaluate the contribution of sarcoplasmic reticulum and mitochondrial organelle dysfunction to regional contractile impairment in post-ischemic stunned myocardium.

January 1, 1995The Showa University Journal of Medical SciencesOpen Access

Alterations in Sarcoplasmic Reticulum and Mitochondrial Functions in Stunned Myocardium : Ralation between Regional Myocardial Function and Biochemical Analyses

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Population

47 mongrel dogs

Comparison

LAD occlusion for 15 min followed by reperfusion… vs Baseline/control state

Design

Preclinical

Follow-up

60 minutes of reperfusion

Authors

HAHirohisa ArataShowa University EGEiichi GeshiShowa University HIHaruhiko IshiokaNational Center for Global Health and Medicine

Discussion

Member takes

Implication

Animal data link SR and mitochondrial dysfunction to myocardial stunning; leaves open translation to human post-ischemic recovery.

Key Points

To evaluate the contribution of sarcoplasmic reticulum and mitochondrial organelle dysfunction to regional contractile impairment in post-ischemic stunned myocardium.
Subjected 47 mongrel dogs to 15 minutes of left anterior descending coronary artery occlusion followed by 60 minutes of reperfusion.
Continuously monitored systemic hemodynamics, coronary blood flow, regional myocardial blood flow, and segment shortening.
Assessed sarcoplasmic reticulum Ca-ATPase activity, mitochondrial respiration, and protein integrity via biochemical assays and SDS-gel electrophoresis.
Segment shortening fell to -22% of baseline during 15-minute ischemia and remained depressed at 58% at 60 minutes of reperfusion despite transient reactive hyperemia reaching 370% of baseline.
Sarcoplasmic reticulum Ca-ATPase activity decreased to 57% during ischemia and recovered to 78% after 60 minutes of reperfusion, while mitochondrial state III respiration recovered to only 77%.
Both Ca-ATPase activity and mitochondrial state III respiration correlated positively with segment shortening, with electrophoresis showing no irreversible structural protein degradation.

Structured PICO

Population

47 mongrel dogs

Intervention

LAD occlusion for 15 min followed by reperfusion for 60 min

Comparator

Baseline/control state

Outcome

Sarcoplasmic reticulum (SR) and mitochondria (Mt) functions, and segment shortening (SS)surrogate

Contractile dysfunction after brief ischemia (stunned myocardium) is related to transient dysfunction of intracellular microorgans like the sarcoplasmic reticulum and mitochondria.

Cite This Study

Arata et al. (1995) studied this question.

synapsesocial.com/papers/6a780f2e7d04941475ae8fcb https://doi.org/10.15369/sujms1989.7.83

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