Key Points
- Investigate the nuclear localisation of class V myosins and determine whether myosin Vb interacts with the RNA polymerase I transcription machinery.
- Examined the nuclear and nucleolar distribution of myosin Va and myosin Vb using paralog-specific antibodies.
- Treated cells with RNA polymerase I inhibitors to assess alterations in nucleolar protein localisation.
- Performed co-immunoprecipitation experiments to evaluate physical interactions between myosin Vb, RNA polymerase I, and actin.
- Myosin Vb localized to nucleoli and co-localized with RNA polymerase I and newly synthesized ribosomal RNA, whereas myosin Va remained largely excluded from nucleoli.
- Inhibition of RNA polymerase I specifically altered the nucleolar distribution of myosin Vb while leaving myosin Va unaffected.
- Co-immunoprecipitation demonstrated that myosin Vb physically associates with actin and the RNA polymerase I transcription complex.
Structured PICO
PPopulationMammalian cells
IInterventionRNA polymerase I inhibitors
OOutcomeLocalisation of myosin Vb and association with RNA polymerase I
Myosin Vb localizes to nucleoli and physically associates with RNA polymerase I, suggesting a role in rRNA transcription.