Key result
Absence of myosin-Va in primary fibroblasts from null mutant mice significantly altered RNA distribution and beta-actin mRNA localization compared to wild-type controls.
Myosin-Va is involved in a transient transport or tethering function for RNA in the perinuclear region soon after synthesis.
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Myosin-Va may mediate early perinuclear RNA tethering in fibroblasts; extends cytoskeletal mRNA transport data but remains hypothesis-generating.
Myosin-Va has been shown to have multiple functions in a variety of cell types, including a role in RNA transport in neurons. Using primary cultures of cells from organs of young dilute-lethal (Myo5a(d-l)/Myo5a(d-l)) null mutant mice and wild-type controls, we show that in some, but not all, tissues, RNA distribution is dramatically different in the homozygous null mutant cells. The dependence of RNA localization on myosin-Va correlates with the relative abundance of the brain-specific splicing pattern of the myosin-Va tail. We also show that myosin-Va is involved in RNA localization soon after synthesis, because the effects of its absence are diminished for RNAs that are more than 30 min old. Finally, we show that localization of beta-actin mRNA is significantly changed by the absence of myosin-Va. These results suggest that myosin-Va is involved in a transient transport or tethering function in the perinuclear region.
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Salerno et al. (2008) studied this question. Myosin-Va null mutation vs. Wild-type controls was evaluated on RNA distribution and localization. Absence of myosin-Va in primary fibroblasts from null mutant mice significantly altered RNA distribution and beta-actin mRNA localization compared to wild-type controls.
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