September 18, 2002BloodOpen Access

P2X1-mediated activation of extracellular signal-regulated kinase 2 contributes to platelet secretion and aggregation induced by collagen

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Population

Washed apyrase-treated platelets and P2X(1)-transfected HEK293 cells

Comparison

alpha,beta-methylene ATP, collagen, and pathway… vs Untreated cells, ADP, or cells expressing…

Design

Preclinical

Authors

COCécile OuryGeneral Cardiology ETEmese Tóth-ZsámbokiSemmelweis University JVJos VermylenUniversità Cattolica del Sacro Cuore

Discussion

Member takes

Implication

P2X1 may modulate mild collagen-induced platelet responses in animals; leaves open its therapeutic targeting in human thrombosis.

Structured PICO

Population

Washed apyrase-treated platelets and P2X(1)-transfected HEK293 cells

Intervention

alpha,beta-methylene ATP, collagen, and pathway inhibitors (U-0126, EGTA, GF109203-X)

Comparator

Untreated cells, ADP, or cells expressing mutated P2X(1)delL nonfunctional channels

Outcome

ERK2 phosphorylation, ATP secretion, and platelet aggregationsurrogate

Mild platelet stimulation with collagen releases ATP, which activates the P2X1-PKC-ERK2 pathway to enhance degranulation and complete platelet aggregation.

Cite This Study

Oury et al. (2002) studied this question.

synapsesocial.com/papers/6a70ad402163a0a01bc4e51d https://doi.org/10.1182/blood-2002-03-0812

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