What question did this study set out to answer?

To evaluate the translation, granule accumulation, and reversibility of mouse mast cell protease 2 (mMCP-2) mRNA and protein in cultured bone marrow-derived mast cells exposed to interleukin-10.

September 1, 1993The Journal of Immunology

Reversible expression of mouse mast cell protease 2 mRNA and protein in cultured mast cells exposed to IL-10.

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Authors

NGN. P. GhildyalCentral Food Technological Research Institute DFDS FriendUniversity of Iowa CNC.F. NicodemusBrigham and Women's Hospital

Discussion

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Implication

In vitro study demonstrates reversible expression of mast cell protease 2 under interleukin-10 exposure in mouse mast cells, suggesting dynamic microenvironment-driven phenotypic plasticity.

Key Points

To evaluate the translation, granule accumulation, and reversibility of mouse mast cell protease 2 (mMCP-2) mRNA and protein in cultured bone marrow-derived mast cells exposed to interleukin-10.
Cultured BALB/cJ mouse bone marrow progenitor cells in WEHI-3 conditioned medium with or without recombinant IL-10 (rIL-10).
Generated an affinity-purified rabbit antibody against synthetic residues 56 to 71 of mMCP-2 to detect the 28-kDa protease via immunoblotting and immunohistochemistry.
Performed time-course analyses tracking mMCP-2 mRNA and protein levels during rIL-10 stimulation and following cytokine withdrawal.
Exposure to rIL-10 induced high steady-state mMCP-2 mRNA levels within 24 hours, whereas peak accumulation of the 28-kDa protease in secretory granules required 7 days.
Removal of rIL-10 caused a rapid drop in mMCP-2 mRNA within 24 hours, while stored mMCP-2 protein levels persisted before declining around day 5.

Cite This Study

Ghildyal et al. (1993) studied this question.

synapsesocial.com/papers/6a70c875439bab0cabc35249 https://doi.org/10.4049/jimmunol.151.6.3206

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Implication

In vitro study demonstrates reversible expression of mast cell protease 2 under interleukin-10 exposure in mouse mast cells, suggesting dynamic microenvironment-driven phenotypic plasticity.

Reversible expression of mouse mast cell protease 2 mRNA and protein in cultured mast cells exposed to IL-10.

Discussion

Member takes

Implication

Key Points

Cite This Study

Reversible expression of mouse mast cell protease 2 mRNA and protein in cultured mast cells exposed to IL-10.

Discussion

Member takes

Implication

Key Points

Cite This Study

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