What question did this study set out to answer?

This research aims to explore how protein kinase C and intracellular calcium impact the K+ current in guinea pig heart cells.

November 1, 1987AJP Heart and Circulatory Physiology

Intracellular Ca2+ and protein kinase C modulate K+ current in guinea pig heart cells

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Population

Single ventricular cells of guinea pig

Comparison

12-O-tetradecanoylphorbol-13-acetate (TPA, 10 M)… vs Absence of TPA or lower concentrations of Cai2+

Design

Preclinical

Authors

NTNoritsugu TohseSapporo Medical University MKMasaki KameyamaShinshu University HIHiroshi IrisawaDokkyo Medical University

Discussion

Member takes

Implication

May modulate APD via IK in rodent myocytes; leaves open human relevance and clinical translation.

Key Points

This research aims to explore how protein kinase C and intracellular calcium impact the K+ current in guinea pig heart cells.
Used internal-dialysis and whole cell voltage-clamp techniques on guinea pig ventricular cells.
Investigated the effects of TPA (10(-9) M) and diacylglycerol on K+ current amplitude.
Examined the role of Cai2+ concentrations on delayed rectifier K+ current without TPA.
TPA increased K+ current amplitude in the presence of Cai2+ greater than 10(-10) M.
1-oleoyl-2-acetylglycerol mimicked TPA's effect; blocked by a specific PKC inhibitor.
K+ current also increased with higher Cai2+ concentrations, indicating direct modulation by Cai2+ alongside PKC activation.

Structured PICO

Population

Single ventricular cells of guinea pig

Intervention

12-O-tetradecanoylphorbol-13-acetate (TPA, 10(-9) M) or 1-oleoyl-2-acetylglycerol (OAG, 50 micrograms/ml, 125 microM) and varying concentrations of intracellular calcium (Cai2+)

Comparator

Absence of TPA or lower concentrations of Cai2+

Outcome

Amplitude of the delayed rectifier K+ current (IK)surrogate

Intracellular calcium modulates the delayed rectifier K+ current in guinea pig ventricular cells both directly and through the activation of protein kinase C, providing a possible mechanism linking calcium transients to action potential duration.

Cite This Study

Tohse et al. (1987) studied this question.

synapsesocial.com/papers/6a70c9cd2fdcb5703edb6844 https://doi.org/10.1152/ajpheart.1987.253.5.h1321

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Intracellular Ca2+ and protein kinase C modulate K+ current in guinea pig heart cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Intracellular Ca2+ and protein kinase C modulate K+ current in guinea pig heart cells

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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