May 1, 1997The Journal of General PhysiologyOpen Access

Ca2+-induced Ca2+ Release in Chinese Hamster Ovary (CHO) Cells Co-expressing Dihydropyridine and Ryanodine Receptors

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Population

Chinese hamster ovary cells co-expressing ryanodine receptor-Ca2+ release channel and dihydropyridine…

Design

Preclinical

Authors

NSNorio SudaJikei University School of Medicine DFDorothee FranziusKyoto University AFAndrea FleigUniversity of Hawaiʻi at Mānoa

Discussion

Member takes

Implication

Skeletal RyR supports CICR in CHO cells without tight coupling; leaves open the role of T-tubule morphology or accessory proteins in native cardiomyocytes.

Structured PICO

Population

Chinese hamster ovary (CHO) cells co-expressing ryanodine receptor (RyR)-Ca2+ release channel and dihydropyridine receptor (DHPR)-Ca2+ channel (alpha2, beta, gamma subunits and chimeric DHPR alpha(i) subunit)

Intervention

Depolarization in the presence or absence of extracellular Ca2+

Outcome

Ca2+ transient (CaT) and intracellular Ca2+ concentration ([Ca2+]i) kineticssurrogate

The skeletal muscle RyR isoform supports Ca2+-induced Ca2+ release in CHO cells, but functional 'close coupling' requires morphological properties of T-tubules or other proteins.

Cite This Study

Suda et al. (1997) studied this question.

synapsesocial.com/papers/6a70d894a528af2d65c3c665 https://doi.org/10.1085/jgp.109.5.619

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Implication

Skeletal RyR supports CICR in CHO cells without tight coupling; leaves open the role of T-tubule morphology or accessory proteins in native cardiomyocytes.

Ca2+-induced Ca2+ Release in Chinese Hamster Ovary (CHO) Cells Co-expressing Dihydropyridine and Ryanodine Receptors

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Ca2+-induced Ca2+ Release in Chinese Hamster Ovary (CHO) Cells Co-expressing Dihydropyridine and Ryanodine Receptors

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider