Use of Intralipid for kinetic analysis of HDL apoC-III: evidence for a homogeneous kinetic pool of apoC-III in plasma
Population
14 men
Comparison
Intralipid extraction of apoC-III from HDL… vs Untreated HDL apoC-III
Design
Other
Authors
Discussion
Loading...
Member takes
Implication
Simplifies apoC-III kinetic studies; supports a homogeneous VLDL-HDL pool but leaves clinical translation open.
Structured PICO
P
Population
14 men
I
Intervention
Intralipid extraction of apoC-III from HDL before apoprotein separation by isoelectric focusing gel electrophoresis
C
Comparator
Untreated HDL apoC-III
O
Outcome
ApoC-III kinetics (isotopic enrichment curves and fractional catabolic rates) and protein purity (isoleucine-to-leucine ratio)surrogate
Intralipid extraction provides a simple method to isolate apoC-III from HDL with minimal interference, demonstrating that apoC-III kinetics in VLDL and HDL are similar and supporting a single homogeneous pool in plasma.
Cite This Study
Nguyen et al. (2006) studied this question.