Transfer of Apolipoprotein C-III between VLDL and HDL after Intravenous Fat Load

Q: What are the key findings of this study?

Intravenous 10% Intralipid (0.1 g/kg) induced rapid apo C-III transfer from HDL to VLDL within 5 minutes, positively correlating with VLDL triglyceride levels over 60 minutes.

Q: What is the clinical evidence from this study?

Population: Lipoprotein metabolism (healthy controls, hypertriglyceridemia, and liver disease) (n=14). Intervention: 10% Intralipid intravenous injection vs. Baseline (pre-load). Primary outcome: Transfer of apolipoprotein C-III between HDL (d > 1.006) and VLDL (d < 1.006) fractions and its correlation with triglyceride concentration.

Q: What does this research mean for the field?

Intravenous fat loading induces rapid transfer of apolipoprotein C-III from HDL to VLDL within 5 minutes, which correlates positively with VLDL triglyceride concentrations during lipolysis. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Kyosuke Yamamoto; Shunichi KOGA; Yoshiyuki Yamanaga; Sadato FUJII; Tetsuro SHONO

doi:10.5551/jat1973.9.2_163

January 1, 1981The Journal of Japan Atherosclerosis SocietyOpen Access

Transfer of Apolipoprotein C-III between VLDL and HDL after Intravenous Fat Load

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Key result

Intravenous Intralipid induces rapid apo C-III transfer from HDL to VLDL, tracking triglyceride levels.

n=14

Why the study?

The study examined the transfer of apolipoprotein C-III between VLDL and HDL after intravenous and in vitro addition of Intralipid.

Key Points

To investigate the dynamic transfer of apolipoprotein C-III between VLDL and HDL fractions following intravenous and in vitro fat loading with Intralipid.
Fourteen participants (5 healthy controls, 4 with hypertriglyceridemia, 3 with liver disease, and 2 others) received an intravenous injection of 10% Intralipid at 0.1 g/kg, with blood drawn at intervals from 0 to 60 minutes.
In vitro tests evaluated sera incubated with 0 to 400 mg/100 ml of Intralipid at 37°C for 30 minutes.
Lipoproteins were separated by ultracentrifugation, and apolipoprotein C-III concentrations were quantified using electroimmunoassay.
Apolipoprotein C-III transferred rapidly from HDL to VLDL within the first 5 minutes post-injection and returned to the higher-density fraction as triglyceride hydrolysis progressed.
Total serum apolipoprotein C-III concentration remained unchanged over 60 minutes of lipolysis, while apolipoprotein C-III demonstrated a significant positive correlation with triglyceride levels in the VLDL fraction.
The initial 5-minute transfer rate of apolipoprotein C-III from HDL to VLDL correlated inversely with baseline apolipoprotein C-III concentrations in VLDL.

PICO

P

Population

14 fasting subjects (5 healthy controls, 4 with hypertriglyceridemia, 3 with liver disease, and 2 others) evaluated for 60 minutes following an intravenous fat load.

I

Intervention / Comparator

10% Intralipid intravenous injection vs Baseline (pre-load) (0.1 g/kg body weight)

O

Primary Outcome

Transfer of apolipoprotein C-III between HDL (d > 1.006) and VLDL (d < 1.006) fractions and its correlation with triglyceride concentration

Limitations

Small sample size (N=14)
Heterogeneous study population
Single-arm pre-post experimental design without a control group

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Implication

Shows rapid transfer of apolipoprotein C-III between HDL and VLDL during fat loading in humans, indicating close coupling with triglyceride dynamics.

Abstract

A transfer of apolipoprotein C-III (apo C-III) between VLDL and HDL was studied after intravenous and in vitro addition of Intralipid using electroimmunoassay.Apo C-III transferred rapidly from HDL to VLDL during first 5 min after intravenous fat load and returned to VLDL following triglyceride hydrolysis.During lipolysis between 60 min, serum apo C-III level did not change, and the significant positive correlation between apo C-III and TG concentration in VLDL was demonstrated. The transfer rate of apo C-III from HDL to VLDL during first 5 min correlated inversely with the original apo C-III levels in VLDL.Upon addition of intralipid to sera in vitro, the transfer of apo C-III from HDL to VLDL occurred in proportion to the increment of TG concentration in VLDL fraction.From these facts, a close relationship between apo C-III and TG concentration have been demonstrated especially in VLDL fraction with or without TG hydrolysis.

Discussion

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Cite This Study

Yamamoto et al. (1981) studied Lipoprotein metabolism (healthy controls, hypertriglyceridemia, and liver disease) (n=14). 10% Intralipid intravenous injection vs. Baseline (pre-load) was evaluated on Transfer of apolipoprotein C-III between HDL (d > 1.006) and VLDL (d < 1.006) fractions and its correlation with triglyceride concentration. Intravenous 10% Intralipid (0.1 g/kg) induced rapid apo C-III transfer from HDL to VLDL within 5 minutes, positively correlating with VLDL triglyceride levels over 60 minutes.

synapsesocial.com/papers/6aed2fafad8601d0d76affbf https://doi.org/10.5551/jat1973.9.2_163

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Transfer of Apolipoprotein C-III between VLDL and HDL after Intravenous Fat Load

Key Points

PICO

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Transfer of Apolipoprotein C-III between VLDL and HDL after Intravenous Fat Load

Key Points

PICO

Limitations

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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