What question did this study set out to answer?

To map the chromosomal location of the human immune interferon (IFN-gamma) gene.

March 1, 1983The Journal of Experimental MedicineOpen Access

Human immune interferon gene is located on chromosome 12.

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Authors

SNSusan L. NaylorThe University of Texas Health Science Center at San Antonio ASA.Y. SakaguchiUniversity of Southern California TST.B. ShowsBrigham and Women's Hospital

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Implication

Somatic cell hybrid analysis demonstrates localization of the immune interferon gene to chromosome 12 in human cells, highlighting its specific genomic coordinates.

Key Points

To map the chromosomal location of the human immune interferon (IFN-gamma) gene.
Used a cDNA clone (plasmid p69) carrying human IFN-gamma sequences to probe genomic DNA from human-rodent somatic cell hybrids.
Performed Southern blot hybridization on EcoRI-digested DNA across 37 hybrid cell lines to detect human-specific 8.8 and 2.0 kilobase pair fragments.
Correlated the presence of human-specific 8.8 and 2.0 kb IFN-gamma DNA fragments with human chromosome 12 across 37 somatic hybrid cell lines.
Localized the IFN-gamma gene regionally to the p1205 to qter segment using a hybrid cell line containing a partial human chromosome 12.

Cite This Study

Naylor et al. (1983) studied this question.

synapsesocial.com/papers/6a70e631e71d69abee08edc2 https://doi.org/10.1084/jem.157.3.1020

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Implication

Somatic cell hybrid analysis demonstrates localization of the immune interferon gene to chromosome 12 in human cells, highlighting its specific genomic coordinates.

Human immune interferon gene is located on chromosome 12.

Discussion

Member takes

Implication

Key Points

Cite This Study

Human immune interferon gene is located on chromosome 12.

Discussion

Member takes

Implication

Key Points

Cite This Study

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