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May 1, 1982Molecular and Cellular Biology

Human beta interferon gene localization and expression in somatic cell hybrids.

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Authors

PPPaula M. PithaRutgers, The State University of New JerseyDSDoris L. SlatePalo Alto UniversityNRNitin RajAnimal Husbandry and Veterinary Department

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Implication

Somatic cell hybrid analysis demonstrates beta 1 interferon gene localization to chromosome 9 in human-rodent hybrids, suggesting the existence of additional unlinked interferon loci.

Key Points

  • To determine the human chromosomal localization and expression pattern of the beta 1 fibroblast interferon gene.
  • Assayed panels of human/mouse and human/hamster somatic cell hybrids for sequence homology to a beta 1 cDNA clone.
  • Tested both genomic DNA and induced mRNA across hybrid cell lines containing or lacking human chromosome 9, including a line retaining a complex 9;11 translocation.
  • Homology to the beta 1 cDNA clone appeared in genomic DNA and induced mRNA exclusively in hybrid lines containing intact human chromosome 9.
  • Hybrid lines lacking chromosome 9 or retaining a 9;11 translocation showed no beta 1 sequence homology.
  • Induced interferon mRNA that lacked homology to the beta 1 cDNA clone was detected in hybrid lines containing human chromosomes 2 and 5 without chromosome 9.

Cite This Study

Pitha et al. (1982) studied this question.

synapsesocial.com/papers/6a93e52e948b7e3210fecc0bhttps://doi.org/10.1128/mcb.2.5.564
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The Somatic Cell Genetics of Human Interferon: Assignment of Human Interferon Loci to Chromosomes 2 and 51974 · 57 citations
  2. 2Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies.1980 · 338 citations
  3. 3Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.1981 · 147 citations
  4. 4Leukocyte and fibroblast interferon genes are located on human chromosome 9.1981 · 125 citations
  5. 5Evidence for a unique human fibroblast interferon (IFN-β1) chromosomal gene, devoid of intervening sequences1981 · 89 citations