The Proteins of Blood and Subcutaneous Lymph in Dogs

In the course of experiments undertaken to discover the effect of the flow and the composition of lymph on the formation of edema an opportunity has been afforded for comparing the albumin and globulin concentrations of blood serum with those of lymph serum obtained from lymphatics of the extremities of dogs. The comparison forms the basis of this communication. METHODS The dogs used were of mongrel breeds and varied in weight from 15 to 26 kilograms. Lymph was obtained through cannulas inserted into one of the lymphatic trunks just above the ankle of either a front or a hind leg. Cannulization was performed sometimes under ether anes-thesia, sometimes under nembutal anesthesia and sometimes with the aid of a local anesthetic (novocaine) only. With animals under general anesthesia the flow of lymph was promoted by gentle massage of the foot and ankle and passive motion of the extremity. The non-anesthetized