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August 1, 1994AJP Cell Physiology

Voltage-dependent ionic conductances in Chinese hamster ovary cells

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Population

Cultures of Chinese hamster ovary native (CHO-K1) cells

Design

Preclinical

Authors

RSRoman SkrymaCentre National de la Recherche ScientifiqueNPNatalia PrevarskayaUniversité Paris-SudPVPierre VacherUniversité de Bordeaux

Discussion

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Overview

Requires accounting for endogenous currents when using CHO-K1 cells for cardiac ion channel studies; leaves open effects on native myocyte physiology.

Structured PICO

P
Population
Cultures of Chinese hamster ovary native (CHO-K1) cells
I
Intervention
Electrophysiological characterization of voltage-dependent ionic conductances
O
Outcome
Identification and properties of membrane ion conductances before transfection

Native CHO-K1 cells possess endogenous voltage-dependent K+, Na+, and Ca2+ conductances, which must be accounted for when using them as an expression system for exogenous ion channels.

Cite This Study

Skryma et al. (1994) studied this question.

synapsesocial.com/papers/6a70ff4c31a3df8243293b60https://doi.org/10.1152/ajpcell.1994.267.2.c544
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