June 1, 1998Journal of Molecular BiologyOpen Access

Orientation changes of fluorescent probes at five sites on the myosin regulatory light chain during contraction of single skeletal muscle fibres

View Full Paper

Ask AI

Bookmark

Population

Demembranated single skeletal muscle fibres from rabbit psoas muscle, exchanged with mutant chicken skeletal…

Design

Preclinical

Authors

CSCibele Sabido-DavidKing's College London SHSeth C. HopkinsOtsuka (United States)LSLakshmi D. SaraswatUniversity of Vermont

Discussion

Member takes

Implication

Small axial tilts refine cross-bridge models in skeletal muscle; leaves open translation to cardiac myosin function.

Structured PICO

Population

Demembranated single skeletal muscle fibres from rabbit psoas muscle, exchanged with mutant chicken skeletal myosin regulatory light chains (RLC) labelled with acetamidotetramethylrhodamine (ATR)

Intervention

Rapid length steps (active release of 5 nm per half sarcomere or active stretch)

Outcome

Changes in fluorescence polarisation indicating orientation of the RLC region of the myosin headsurrogate

This basic science study demonstrates that during active contraction of skeletal muscle fibers, the myosin regulatory light chain undergoes small axial tilts (less than 5 degrees) in response to rapid length steps.

Cite This Study

Sabido-David et al. (1998) studied this question.

synapsesocial.com/papers/6a71288053da1ffdb507b5af https://doi.org/10.1006/jmbi.1998.1771

View Full Paper

Ask AI

Bookmark

Orientation changes of fluorescent probes at five sites on the myosin regulatory light chain during contraction of single skeletal muscle fibres

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Orientation changes of fluorescent probes at five sites on the myosin regulatory light chain during contraction of single skeletal muscle fibres

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider