Fluctuations in polarized fluorescence: evidence that muscle cross bridges rotate repetitively during contraction.

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Structured PICO

Population

Single glycerinated muscle fibers with myosin subfragment 1 moieties covalently labeled with rhodamine

Intervention

Excitation by polarized 514.5-nm laser light during steady-state activity (active isometric tension)

Comparator

Relaxation or rigor states, or when fluorophore is actin-attached instead of myosin-attached

Outcome

Fluctuations in polarized fluorescence intensity and ratios of polarized intensitiessurrogate

The study provides biophysical evidence using polarized fluorescence that muscle cross bridges rotate repetitively during active contraction.

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Supports cross-bridge rotation in active contraction; leaves open translation to human cardiac mechanics.

Borejdo et al. (1979) studied this question.

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