April 1, 1996Biophysical JournalOpen Access

Orientation changes in myosin regulatory light chains following photorelease of ATP in skinned muscle fibers

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Population

Skinned muscle fibers from rabbit psoas muscle with regulatory light chain from chicken gizzard myosin…

Comparison

Photorelease of ATP (photolysis of caged ATP) vs Photorelease of ADP

Design

Preclinical

Authors

TATaylor AllenOberlin College NLNicholas LingUniversity of Southern California MIMalcolm IrvingHobart and William Smith Colleges

Discussion

Member takes

Implication

Hypothesis-generating for myosin detachment kinetics; leaves open translation to intact or human cardiac muscle.

Structured PICO

Population

Skinned muscle fibers from rabbit psoas muscle with regulatory light chain from chicken gizzard myosin covalently modified with iodoacetamidotetramethylrhodamine

Intervention

Photorelease of ATP (photolysis of caged ATP)

Comparator

Photorelease of ADP

Outcome

Orientation changes in the light-chain region of myosin heads measured by polarized fluorescence (Q parallel and Q perpendicular)surrogate

Photorelease of ATP in skinned muscle fibers demonstrates prompt orientation changes in the myosin light-chain region associated with ATP binding or cross-bridge detachment.

Cite This Study

Allen et al. (1996) studied this question.

synapsesocial.com/papers/6a76b73633def31403adf9db https://doi.org/10.1016/s0006-3495(96)79750-3

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Orientation changes in myosin regulatory light chains following photorelease of ATP in skinned muscle fibers

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Orientation changes in myosin regulatory light chains following photorelease of ATP in skinned muscle fibers

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider