August 1, 1987Journal of Biological ChemistryOpen Access

Proximity and ligand-induced movement of interdomain residues in myosin subfragment 1 containing trapped MgADP and MgPPi probed by multifunctional cross-linking.

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Population

Myosin subfragment 1 containing trapped MgADP or MgPPi

Comparison

Modification with trifunctional photoactivatable… vs Comparison between trapped MgADP and trapped MgPPi

Design

Preclinical

Authors

KRKallikat N. RajasekharanCentral Tuber Crops Research Institute MSM SivaramakrishnanThe University of Texas MD Anderson Cancer Center MBM BurkeUtrecht University

Discussion

Member takes

Implication

Distinguishes nucleotide-specific myosin rigidity in vitro; hypothesis-generating for cardiac cross-bridge mechanics.

Structured PICO

Population

Myosin subfragment 1 containing trapped MgADP or MgPPi

Intervention

Modification with trifunctional photoactivatable reagent 4,4'-bis(N-maleimido)benzophenone and photolysis

Comparator

Comparison between trapped MgADP and trapped MgPPi

Outcome

Extent and course of photoinduced cross-linkingsurrogate

The binding of MgADP versus MgPPi to myosin subfragment 1 induces distinct conformational states, with MgADP creating a more rigid structure compared to the flexible state induced by MgPPi.

Cite This Study

Rajasekharan et al. (1987) studied this question.

synapsesocial.com/papers/6a71432026a7f98052dda4be https://doi.org/10.1016/s0021-9258(18)60945-7

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Proximity and ligand-induced movement of interdomain residues in myosin subfragment 1 containing trapped MgADP and MgPPi probed by multifunctional cross-linking.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Proximity and ligand-induced movement of interdomain residues in myosin subfragment 1 containing trapped MgADP and MgPPi probed by multifunctional cross-linking.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider