February 1, 1982Proceedings of the National Academy of SciencesOpen Access

Tryptic digestion as a probe of myosin S-1 conformation.

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Population

chymotryptic myosin subfragment 1

Design

Preclinical

Authors

AMAndrás MühlrádHebrew University of Jerusalem THTetsu HozumiNagoya City University

Discussion

Member takes

Implication

Preclinical only; leaves open whether myosin hinge flexibility modulates cardiac contractility in vivo.

Structured PICO

Population

chymotryptic myosin subfragment 1

Intervention

Tryptic digestion under varying conditions (pH, temperature, ionic strength, MgATP, F-actin)

Outcome

Generation of the 27,000-dalton NH2-terminal fragment and suppression of digestion routes

The 27,000/50,000-dalton hinge region of the myosin head is a flexible domain whose conformation is sensitive to nucleotides, actin, and ambient factors.

Cite This Study

Mühlrád et al. (1982) studied this question.

synapsesocial.com/papers/6a71432126a7f98052dda4c3 https://doi.org/10.1073/pnas.79.4.958

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Ask AI

Bookmark

Tryptic digestion as a probe of myosin S-1 conformation.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Tryptic digestion as a probe of myosin S-1 conformation.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider