Influence of temperature on the calcium sensitivity of the myofilaments of skinned ventricular muscle from the rabbit.
Population
Skinned cardiac trabeculae from the rabbit right ventricle (diameter, 0.13-0.34 mm)
Comparison
Exposure to different temperatures and… vs Different temperatures compared to each other
Design
Preclinical
Authors
Discussion
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Implication
Cooling reduces myofilament Ca sensitivity without mediating hypothermic inotropy in rabbits; hypothesis-generating for mechanisms, not applicable to patients.
Structured PICO
P
Population
Skinned cardiac trabeculae from the rabbit right ventricle (diameter, 0.13-0.34 mm)
I
Intervention
Exposure to different temperatures (36, 29, 22, 15, 8, and 1 degree C) and Ca-containing solutions (pCa 6.3-4.0)
C
Comparator
Different temperatures compared to each other (e.g., 22 degrees C as reference for maximum tension)
O
Outcome
Steady-state myofilament Ca sensitivity (pCa corresponding to half maximal tension) and maximum tension (Cmax)surrogate
Cooling decreases tension at all levels of calcium concentration, suggesting changes in myofilament calcium sensitivity do not play a role in the large hypothermic inotropy seen in rabbit ventricular muscle.
Cite This Study
Harrison et al. (1989) studied this question.