February 1, 1990AJP Cell Physiology
Modification of temperature dependence of myofilament Ca sensitivity by troponin C replacement
Why the study?
Does replacement of endogenous troponin C with skeletal troponin C modify the temperature dependence of myofilament Ca sensitivity in rat right ventricular trabeculae?
Population
Chemically skinned right ventricular trabeculae from the rat heart (n=14)
Comparison
Extraction of endogenous troponin C with EDTA… vs Native state or reconstitution with bovine…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
TnC isoform dictates cooling effects on myofilament Ca sensitivity in rat trabeculae; leaves open translational relevance to human myocardium.
Structured PICO
Does replacement of endogenous troponin C with skeletal troponin C modify the temperature dependence of myofilament Ca sensitivity in rat right ventricular trabeculae?
P
Population
Chemically skinned right ventricular trabeculae from the rat heart (n=14)
I
Intervention
Extraction of endogenous troponin C (TnC) with EDTA and replacement with rabbit fast-twitch skeletal TnC
C
Comparator
Native state (endogenous TnC) or reconstitution with bovine cardiac TnC
O
Outcome
Temperature dependence of myofilament Ca sensitivity (shift in pCa generating half-maximal tension [K1/2] when cooling from 22 to 8 degrees C)surrogate
Calcium binding to troponin C plays an important role in determining the temperature dependence of myofilament calcium sensitivity in cardiac muscle.
Cite This Study
Harrison et al. (1990) studied this question.