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November 10, 1998Circulation

Direct Intramuscular Injection of Plasmid DNA Encoding Angiopoietin-1 but not Angiopoietin-2 Augments Revascularization in the Rabbit Ischemic Hindlimb

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Why the study?

Does intramuscular injection of plasmid DNA encoding Ang1 or Ang2 improve collateral vessel development and limb perfusion in a rabbit model of hindlimb ischemia?

Population

Rabbit model of hindlimb ischemia (n=48)

Comparison

Intramuscular injection of plasmid DNA encoding… vs Intramuscular injection of empty plasmid control…

Design

Preclinical

Follow-up

30 days

Authors

KSKou‐Gi ShyuFu Jen Catholic UniversityOMOrit ManorTel Aviv UniversityMMMeredith MagnerTufts University

Discussion

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Implication

Ang1 gene transfer may enhance perfusion in experimental limb ischemia; leaves open translation to human therapeutic angiogenesis.

Structured PICO

Does intramuscular injection of plasmid DNA encoding Ang1 or Ang2 improve collateral vessel development and limb perfusion in a rabbit model of hindlimb ischemia?

P
Population
Rabbit model of hindlimb ischemia (n=48)
I
Intervention
Intramuscular injection of plasmid DNA encoding Angiopoietin-1 (pAng1*) or Angiopoietin-2 (pAng2)
C
Comparator
Intramuscular injection of empty plasmid control (pJFE or pcDNA3) or saline
O
Outcome
Collateral vessel development and limb perfusion (assessed by calf blood pressure ratio, angiographic score, maximal blood flow, and capillary density) at 30 dayssurrogate

Gene transfer of Angiopoietin-1, but not Angiopoietin-2, enhances collateral vessel formation and limb perfusion in a rabbit model of hindlimb ischemia, suggesting a potential therapeutic strategy for tissue ischemia.

Cite This Study

Shyu et al. (1998) studied this question.

synapsesocial.com/papers/6a7148af3ce530166bc30e50https://doi.org/10.1161/01.cir.98.19.2081
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Also Consider

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  3. 3Gene Transfer in Regenerating Muscle1994 · 174 citations
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