What question did this study set out to answer?

To identify the specific receptor subtype and downstream second messenger pathways mediating angiotensin II-induced hypertrophic growth in cardiac myocytes.

June 1, 1994AJP Heart and Circulatory Physiology

Hypertrophic growth of cultured neonatal rat heart cells mediated by type 1 angiotensin II receptor

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Population

Primary cultures of neonatal rat cardiac myocytes

Comparison

Angiotensin II exposure, with or without losartan vs Control (untreated cells)

Design

Preclinical

Authors

SMSetsuya MiyataIwate Medical University THTakashi HanedaHyogo Medical University

Discussion

Member takes

Implication

AT1 blockade prevents ANG II-driven hypertrophy in neonatal rat myocytes; supports mechanistic role but leaves open clinical translation.

Key Points

To identify the specific receptor subtype and downstream second messenger pathways mediating angiotensin II-induced hypertrophic growth in cardiac myocytes.
Treated primary cultures of neonatal rat cardiac myocytes with angiotensin II (ANG II).
Measured protein-to-DNA and RNA-to-DNA ratios, protein synthesis rates, intracellular calcium, protein kinase C (PKC) activity, and c-Fos mRNA expression in the presence or absence of the AT1 receptor antagonist losartan.
ANG II significantly increased protein-to-DNA and RNA-to-DNA ratios and accelerated protein synthesis rates by 24.9%.
ANG II induced a transient elevation in intracellular free calcium, altered cytosolic and particulate PKC activity, and increased c-Fos mRNA expression; all stimulatory effects were blocked by losartan.

Structured PICO

Population

Primary cultures of neonatal rat cardiac myocytes

Intervention

Angiotensin II (ANG II) exposure, with or without losartan (type 1 angiotensin II receptor antagonist)

Comparator

Control (untreated cells)

Outcome

Hypertrophic growth (measured by protein-to-DNA and RNA-to-DNA ratios, protein synthesis rates, intracellular free calcium, protein kinase C activity, and c-Fos mRNA content)surrogate

Angiotensin II induces hypertrophic growth in neonatal cardiac myocytes, an effect that is mediated at least in part through the AT1 receptor.

Cite This Study

Miyata et al. (1994) studied this question.

synapsesocial.com/papers/6a714bc575498292b70ac77d https://doi.org/10.1152/ajpheart.1994.266.6.h2443

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Hypertrophic growth of cultured neonatal rat heart cells mediated by type 1 angiotensin II receptor

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Hypertrophic growth of cultured neonatal rat heart cells mediated by type 1 angiotensin II receptor

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider