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February 1, 1988American Journal of Clinical Pathology

A Rapid Assay for Platelet Thromboxane Production and Its Use in Assessing Prior Aspirin Ingestion

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Why the study?

Does a rapid assay for platelet thromboxane production accurately detect prior aspirin ingestion?

Population

Normal controls and subjects ingesting various doses of aspirin

Comparison

Single dose of aspirin vs Normal controls without aspirin ingestion

Design

Other

Follow-up

Up to 5 days

Authors

ACAnn Marie ConnorOur Lady of Lourdes HospitalMLMichael LaposataThe University of Texas Medical Branch at Galveston

Discussion

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Implication

Thromboxane assay may detect recent aspirin ingestion in practice; leaves open prospective validation for cyclooxygenase monitoring.

Key Points

  • Develop a simple, rapid, and cost-effective assay for measuring platelet thromboxane synthesis to detect cyclooxygenase defects and recent aspirin ingestion.
  • Quantified the conversion of 14C-arachidonate to 14C-thromboxane via thin-layer chromatography and scintillation counting to establish a thromboxane index normalized to platelet count.
  • Measured thromboxane production 12 to 16 hours after administering single doses of aspirin (41, 81, 163, 325, and 650 mg; n=3 per group) and compared values to unexposed controls (n=25).
  • Tracked the longitudinal recovery of thromboxane synthetic capacity over several days in four subjects after a single 650-mg aspirin dose.
  • Healthy control subjects exhibited a baseline thromboxane index of 2.41 ± 0.77 (mean ± SD, n=25).
  • Thromboxane synthesis was completely blocked 12–16 hours post-dose by 650 mg (0.27 ± 0.14), 325 mg (0.29 ± 0.18), and 163 mg (0.13 ± 0.13), with partial inhibition observed at 81 mg (0.41 ± 0.46) and 41 mg (1.41 ± 0.75).
  • Thromboxane synthetic capacity returned to normal levels by the fifth day after ingestion in three of the four longitudinally monitored subjects.

Structured PICO

Does a rapid assay for platelet thromboxane production accurately detect prior aspirin ingestion?

P
Population
Normal controls (n=25) and subjects ingesting various doses of aspirin (n=3 for each of 5 doses, n=4 for longitudinal follow-up)
I
Intervention
Single dose of aspirin (650 mg, 325 mg, 163 mg, 81 mg, or 41 mg)
C
Comparator
Normal controls without aspirin ingestion
O
Outcome
Platelet thromboxane production (measured as thromboxane index)surrogate

A simple, rapid assay for platelet thromboxane production can effectively detect prior aspirin ingestion and assess cyclooxygenase function.

Cite This Study

Connor et al. (1988) studied this question.

synapsesocial.com/papers/6a7153ca35aa2c282ce2a148https://doi.org/10.1093/ajcp/89.2.216
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Radioimmunoassay for plasma thromboxane B2.1981 · 21 citations
  2. 2Impaired Thromboxane Production by Newly Formed Platelets after Aspirin Administration to Thrombocytopenic Rats1980 · 25 citations
  3. 3Functional Testing Methods for the Antiplatelet Effects of Aspirin2011 · 36 citations
  4. 4Inhibition of thromboxane formation in vivo and ex vivo: implications for therapy with platelet inhibitory drugs1987 · 374 citations
  5. 5Inhibition of Prostacyclin and Platelet Thromboxane A2after Low-Dose Aspirin1981 · 272 citations