Specificity of action of guanine nucleotide-binding regulatory protein subunits on the cardiac muscarinic K+ channel.

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Population

Embryonic chicken and neonatal rat atrial patches

Comparison

Purified beta gamma subunits and activated alpha… vs Transducin beta gamma, unactivated alpha…

Design

Preclinical

Authors

Discussion

Caution against clinical translation from animal patches; leaves open precise G-protein roles in human cardiac signaling.

Population

Embryonic chicken and neonatal rat atrial patches

Intervention

Purified beta gamma subunits (from human placenta or bovine brain) and activated alpha subunits (from human erythrocytes or bovine brain)

Comparator

Transducin beta gamma, unactivated alpha subunits, detergent (CHAPS) alone, buffer, or heat-inactivated beta gamma

Outcome

Activation (opening) of the cardiac muscarinic K+ channel (IK.ACh)surrogate

Both beta gamma and alpha subunits of G proteins can activate the cardiac muscarinic K+ channel, suggesting a complex mechanism of G protein action.

Logothetis et al. (1988) studied this question.

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