What question did this study set out to answer?

To determine the functional relationship between mitogen-activated protein kinase (MAPK) activation and phospholipase D (PLD) signaling in A7r5 rat vascular smooth muscle cells.

September 1, 1994Journal of Biological ChemistryOpen Access

Activations of mitogen-activated protein kinases and phospholipase D in A7r5 vascular smooth muscle cells.

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Authors

LJLinda G. JonesUniversity of Arkansas for Medical Sciences KEKrishna M. EllaBharat Biotech (India)CBCynthia D. BradshawMedical University of South Carolina

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Implication

In vitro study reveals independent activation of MAP kinases and phospholipase D in vascular smooth muscle cells, indicating separate pathways during vasopressin signaling.

Key Points

To determine the functional relationship between mitogen-activated protein kinase (MAPK) activation and phospholipase D (PLD) signaling in A7r5 rat vascular smooth muscle cells.
Treated cultured A7r5 rat vascular smooth muscle cells with vasopressin, phorbol ester (PMA), serum, bacterial PLD, phosphatidic acid, or lysophosphatidic acid.
Assessed the activation kinetics of Erk-1, Erk-2, phospholipase C, and PLD, alongside general protein phosphorylation patterns and DNA synthesis.
Vasopressin, PMA, and serum activated Erk-1 and Erk-2, while vasopressin and PMA both stimulated maximal PLD activity within 5 minutes.
Bacterial PLD, phosphatidic acid, and lysophosphatidic acid elevated protein phosphorylation but failed to activate Erk-1 and Erk-2.
Serum and vasopressin stimulated DNA synthesis after exposures exceeding 30 minutes, whereas acute PLD, PMA, phosphatidic acid, and lysophosphatidic acid did not promote mitogenesis.

Cite This Study

Jones et al. (1994) studied this question.

synapsesocial.com/papers/6a71663235aa2c282ce2b449 https://doi.org/10.1016/s0021-9258(17)31585-5

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Implication

In vitro study reveals independent activation of MAP kinases and phospholipase D in vascular smooth muscle cells, indicating separate pathways during vasopressin signaling.

Activations of mitogen-activated protein kinases and phospholipase D in A7r5 vascular smooth muscle cells.

Discussion

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Implication

Key Points

Cite This Study

Activations of mitogen-activated protein kinases and phospholipase D in A7r5 vascular smooth muscle cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

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