Key result
In MDCK-D1 cells, alpha-1-adrenergic receptors stimulate arachidonic acid release through phosphorylation-dependent activation of the 85-kD cytosolic phospholipase A2 by MAP kinase subsequent to PKC activation.
This study elucidates a mechanism by which G protein-coupled receptors stimulate arachidonic acid release via PKC and MAP kinase-dependent activation of cPLA2.
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Should not alter clinical practice; leaves open in vivo validation of this cPLA2 pathway in cardiovascular models.
Xing et al. (1996) studied In vitro cell model (MDCK-D1 cells). Epinephrine and PMA vs. Unstimulated cells or inhibitor-treated cells was evaluated on Arachidonic acid release and cPLA2 activation. In MDCK-D1 cells, alpha-1-adrenergic receptors stimulate arachidonic acid release through phosphorylation-dependent activation of the 85-kD cytosolic phospholipase A2 by MAP kinase subsequent to PKC activation.