March 1, 1986Proceedings of the National Academy of SciencesOpen Access

A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.

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Population

Cultured monkey cells and HeLa cells

Comparison

Transfection with a chimeric plasmid containing… vs Growth at 33.5 degrees C

Design

Preclinical

Authors

BSB L SemlerUniversity of California, Irvine VJVictoria H. JohnsonUniversity of California, Irvine STS. TracyUniversity of Nebraska Medical Center

Discussion

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Implication

Does not support clinical translation; leaves open the role of 5' noncoding sequences in enterovirus replication.

Structured PICO

Population

Cultured monkey cells and HeLa cells

Intervention

Transfection with a chimeric plasmid containing a 405-nucleotide fragment from the 5' noncoding region of coxsackievirus B3 inserted into an infectious cDNA copy of the poliovirus RNA genome

Comparator

Growth at 33.5 degrees C

Outcome

Viral growth, viral protein levels, and viral RNA levels at different temperaturessurrogate

Insertion of a portion of the coxsackievirus genome into the poliovirus genome produces a temperature-sensitive recombinant virus, suggesting RNA sequences in the 5' noncoding region may be involved in replicase recognition or ribosome binding.

Cite This Study

Semler et al. (1986) studied this question.

synapsesocial.com/papers/6a716670f44fa9f079df3284 https://doi.org/10.1073/pnas.83.6.1777

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A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider