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February 1, 1989Journal of VirologyOpen Access

The deletion of 41 proximal nucleotides reverts a poliovirus mutant containing a temperature-sensitive lesion in the 5' noncoding region of genomic RNA

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Population

Infectious cDNA copy of the poliovirus RNA genome transfected into COS-1 and HeLa cells

Comparison

Deletion of nucleotides 221 to 224 and… vs Wild-type poliovirus RNA

Design

Preclinical

Authors

SDSandra L. DildineUniversity of California, IrvineBSBert L. SemlerUniversity of California, Irvine

Discussion

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Implication

Stem-loop deletion may be nonessential for poliovirus replication in vitro; leaves open roles in pathogenesis or attenuation strategies.

Structured PICO

P
Population
Infectious cDNA copy of the poliovirus RNA genome transfected into COS-1 and HeLa cells
I
Intervention
Deletion of nucleotides 221 to 224 (mutant 220D1) and subsequent deletion of 41 proximal nucleotides (overall deletion 184 to 228, revertant R2)
C
Comparator
Wild-type poliovirus RNA
O
Outcome
Viral growth, virus-specific protein and RNA synthesis, and host cell protein synthesis at different temperatures (33, 37, and 39 degrees C)

Deletion of an entire predicted stem-loop structure in the 5' noncoding region of poliovirus RNA reverts a temperature-sensitive mutation, demonstrating these sequences are nonessential for viral growth in HeLa cells.

Cite This Study

Dildine et al. (1989) studied this question.

synapsesocial.com/papers/6a87d4049842aaac2ffb129bhttps://doi.org/10.1128/jvi.63.2.847-862.1989
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1An RNA sequence of hundreds of nucleotides at the 5' end of poliovirus RNA is involved in allowing viral protein synthesis1988 · 173 citations
  2. 2An internal 5'-noncoding region required for translation of poliovirus RNA in vitro1988 · 80 citations
  3. 3Site-directed mutagenesis of proteinase 3C results in a poliovirus deficient in synthesis of viral RNA polymerase1987 · 58 citations
  4. 4Cap-independent translation of poliovirus mRNA is conferred by sequence elements within the 5' noncoding region.1988 · 271 citations
  5. 5Cap-Independent Translation of Poliovirus mRNA is Conferred by Sequence Elements within the 5′ Noncoding Region1988 · 136 citations