Isolation of poliovirus 2C mutants defective in viral RNA synthesis
Population
Poliovirus mutants with oligonucleotide linker insertion in the 2C-coding region, tested on HeLa cell…
Design
Preclinical
Authors
Discussion
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Implication
May inform 2C-targeted antivirals; hypothesis-generating and requires validation beyond animal models.
Structured PICO
P
Population
Poliovirus mutants (2C-31 and 2C-32) with oligonucleotide linker insertion in the 2C-coding region, tested on HeLa cell monolayers
I
Intervention
Temperature shift experiments and trans complementation
O
Outcome
Viral RNA synthesissurrogate
Protein 2C is continuously required for poliovirus RNA synthesis and can be provided in trans.
Cite This Study
Li et al. (1988) studied this question.