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February 1, 1994Journal of VirologyOpen Access

Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis

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Population

Poliovirus RNA-dependent RNA polymerase 3D (27 mutagenized cDNA clones)

Design

Preclinical

Authors

SDScott E. DiamondUniversity of KentuckyKKKarla KirkegaardStanford University

Discussion

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Implication

Offers genetic tool for probing poliovirus replication; leaves open validation of replication complex components in vivo.

Structured PICO

P
Population
Poliovirus RNA-dependent RNA polymerase 3D (27 mutagenized cDNA clones)
I
Intervention
Clustered charged-to-alanine mutagenesis
O
Outcome
Generation of temperature-sensitive (ts) phenotypes and defects in RNA synthesissurrogate

Clustered charged-to-alanine mutagenesis of poliovirus RNA polymerase successfully generated temperature-sensitive mutants defective in RNA synthesis, providing a tool for identifying viral replication complex components.

Cite This Study

Diamond et al. (1994) studied this question.

synapsesocial.com/papers/6a7c911ef1688b70c0610e2fhttps://doi.org/10.1128/jvi.68.2.863-876.1994
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