Key result
The temperature-sensitive mutant tsB9 accumulated only 25% of the wild-type amount of virus-specific RNA and failed to synthesize mature 35S RNA at the restrictive temperature of 39 degrees C.
The tsB9 temperature-sensitive mutant of poliovirus type 1 is defective in mature RNA synthesis at restrictive temperatures but still inhibits host protein synthesis.
tsB9 separates RNA maturation from host shutoff; leaves open mechanistic dissection of poliovirus attenuation.
We isolated six temperature-sensitive mutants of poliovirus type 1 (Mahoney) by hydroxylamine mutagenesis and replica plating at 31, 33 (permissive), and 39 degrees C (restrictive). One of these mutants, designated tsB9, was chosen for more detailed examination. tsB9 accumulated 25% of the wild-type amount of virus-specific RNA at the restrictive temperature. We found that tsB9 was not able to synthesize mature, 35S single-stranded RNA at the restrictive temperature. In spite of the absence of significant RNA synthesis, tsB9 retained the ability to inhibit host protein synthesis during infection at 39 degrees C at about the same rate as wild-type virus.
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Hewlett et al. (1982) studied Poliovirus type 1. Temperature-sensitive mutant tsB9 vs. Wild-type poliovirus type 1 (Mahoney) was evaluated on Virus-specific RNA accumulation at restrictive temperature (39 degrees C). The temperature-sensitive mutant tsB9 accumulated only 25% of the wild-type amount of virus-specific RNA and failed to synthesize mature 35S RNA at the restrictive temperature of 39 degrees C.
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