What question did this study set out to answer?

The study aims to investigate the ontogeny of Na+/Ca2+ exchange current density in cardiac myocytes from rabbits and guinea pigs.

April 1, 1995AJP Heart and Circulatory Physiology

Na+/Ca2+ exchange current density in cardiac myocytes from rabbits and guinea pigs during postnatal development

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Population

Single freshly isolated cardiac myocytes from developing rabbits (1-22 days old) and newborn/adult guinea pigs

Comparison

Whole cell voltage clamp techniques using a… vs Adult guinea pig myocytes; age-based comparison…

Design

Preclinical

Authors

MAM. ArtmanCalifornia Institute of Technology HIH. IchikawaBridgestone (Japan)MAMetin AvkiranBritish Heart Foundation

Discussion

Member takes

Implication

Species differences in neonatal Na+/Ca2+ exchange urge caution extrapolating animal models; leaves open human developmental Ca2+ handling studies.

Key Points

The study aims to investigate the ontogeny of Na+/Ca2+ exchange current density in cardiac myocytes from rabbits and guinea pigs.
Characterized Na+/Ca2+ exchange current using whole cell voltage clamp in isolated cardiac myocytes from rabbits and guinea pigs.
Implemented voltage protocol ranging from -120 to +60 mV to measure exchange currents.
Blocked various currents with Ba2+, Cs+, tetraethylammonium, D-600, and ouabain.
Na+/Ca2+ exchange current density is highest in rabbits at 1-4 days old then decreases rapidly over three weeks.
In guinea pigs, current density at birth is similar to that in adults, indicating functional exchange at birth.
Findings suggest different roles of Na+/Ca2+ exchange in rabbits and guinea pigs due to variations in SR development.

Structured PICO

Population

Single freshly isolated cardiac myocytes from developing rabbits (1-22 days old) and newborn/adult guinea pigs

Intervention

Whole cell voltage clamp techniques using a slow-ramp voltage protocol (-120 to +60 mV, 0.09 V/s) in the presence of Ba2+, Cs+, tetraethylammonia, D-600, and ouabain

Comparator

Adult guinea pig myocytes (for guinea pigs); age-based comparison (1-4 days vs older) for rabbits

Outcome

Ni(2+)-sensitive Na+/Ca2+ exchange current densitysurrogate

The study demonstrates that Na+/Ca2+ exchange assumes a relatively greater role in newborn animals with a sparse sarcoplasmic reticulum, such as rabbits, compared to those with a more extensive SR like guinea pigs.

Cite This Study

Artman et al. (1995) studied this question.

synapsesocial.com/papers/6a716b1f2163a0a01bc59023 https://doi.org/10.1152/ajpheart.1995.268.4.h1714

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Implication

Species differences in neonatal Na+/Ca2+ exchange urge caution extrapolating animal models; leaves open human developmental Ca2+ handling studies.

Na+/Ca2+ exchange current density in cardiac myocytes from rabbits and guinea pigs during postnatal development

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Na+/Ca2+ exchange current density in cardiac myocytes from rabbits and guinea pigs during postnatal development

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider